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专利名称：Method and kit for determining the genome
integrity and/or the quality of a library ofdna sequences obtained by deterministicrestriction site whole genome amplification
发明人：Klein, Christoph Andreas,Polzer, Bernhard
Michael,Manaresi, Nicolò
申请号：EP13195770.6申请日：20131204公开号：EP2881739A1公开日：20150610
专利附图：
摘要：The present invention relates to a method for determining the integrity of thegenome of a sample and/or the quality of a library of DNA sequences obtained bydeterministic restriction site whole genome amplification (DRS-WGA) of the genome ofthe sample comprising the steps of: (a) providing the library of DNA sequences; (b)amplifying the library of DNA sequences by PCR using at least one first primer pair whichhybridises to a DNA sequence of the library having a length from 1000 bp to 5000 bp andcorresponding to a sequence of the genome located on a first chromosome arm, thestep of amplifying giving rise to a first PCR product from 50 to 1000 bp; (c) detecting thefirst PCR product; (d) correlating the presence of the first PCR product with the integrityof the genome and/or the quality of the library of DNA sequences. The present inventionfurther relates to a related kit and uses thereof.
申请人：Silicon Biosystems S.p.A.,Fraunhofer-Gesellschaft zur Förderung derangewandten Forschung e.V.
地址：Via dei Lapidari 12 Bologna IT,Hansastrasse 27c 80686 München DE
国籍：IT,DE
代理机构：Mangini, Simone
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