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专利名称：COMBINATORIAL LIBRARIES OF PROTEINS
HAVING THE SCAFFOLD STRUCTURE OF C-TYPE LECTIN-LIKE DOMAINS
发明人：ETZERODT, Michael,HOLTET, Thor,
Las,GRAVERSEN, Niels, Jonas,
Heilskov,THOGERSEN, Hans, Christian
申请号：EP01270552.1申请日：20011213公开号：EP1341912A2公开日：20030910
摘要：A novel family of protein libraries comprising CTLDs (C-type Lectin-LikeDomains) in which internal polypeptide loop-regions lining the ligand binding sites inCTLDs have been replaced with ensembles of completely or partially randomisedpolypeptide segments. Tetranectin CTLDs were chosen as framework for the preferredembodiment of the invention; and versatile phagemid vectors useful in the generationand manipulation of human and murine tetranectin CTLD libraries are disclosed as part ofthis invention. Tetranectin CTLDs in monomeric as well as in trimeric form are efficientlydisplayed as gene III fusions in fully functional form by the recombinant fd phage displayvector. CTLD derivatives with affinity for new ligands may readily be isolated fromlibraries of vectors displaying CTLDs, in which loop-regions have been randomised, usingone or more rounds of enrichment by screening or selection followed by amplification ofthe enriched subpopulation in each round. The efficiency with which protein productscontaining CTLDs with new binding properties can be produced, e.g. by bacterial
expression and in vitro refolding, in mono-, tri-, or multimeric formats provides importantadvantages in terms of simplicity, cost and efficiency of generation, production anddiagnostic or therapeutic applications in comparison to recombinant antibody derivatives.
申请人：Borean Pharma A/S
地址：Science Park Aarhus,Gustav Wieds Vej 10 8000 Aarhus C DK
国籍：DK
代理机构：Nyeng, Joergen
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