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Compositions for DNA amplification, synthesis, and

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专利名称:Compositions for DNA amplification,

synthesis, and mutagenesis

发明人:Holly Hurlbut Hogrefe,Michael C.

Borns,Michael L. Muhich

申请号:US11067304申请日:20050225

公开号:US200602462A1公开日:20061102

摘要:This invention provides compositions comprising a thermostable non-proofreading DNA polymerase, a thermostable proofreading DNA polymerase, and afactor that substantially inhibits the incorporation of undesired nucleotides or analogsthereof into a DNA polymer. The compositions may further comprise a buffer thatenhances a polymerization reaction involving DNA polymerases. The invention alsoprovides various methods of amplifying, synthesizing, or mutagenizing nucleic acids ofinterest using these novel compositions. Kits that comprise the compositions are alsoprovided for amplifying, synthesizing, and mutagenizing nucleic acids.

申请人:Holly Hurlbut Hogrefe,Michael C. Borns,Michael L. Muhich

地址:San Diego CA US,Escondido CA US,Olivenhain CA US

国籍:US,US,US

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